Roger Y. Tsien

141.6k citations

321 papers · 111.4k indexed · 82 hit papers · h-index 151

Molecular Biology top 0.01%
Cellular and Molecular Neuroscience top 0.01%
Biophysics top 0.01%
Cell Biology top 0.01%
Biomedical Engineering top 0.1%

Co-authors: Martin Poenie Grzegorz Grynkiewicz Stephen Adams T J Rink Tullio Pozzan David A. Zacharias Paul Steinbach Roger Heim
Topics: Advanced Fluorescence Microscopy Techniques (77 papers)Photoreceptor and optogenetics research (50 papers)bioluminescence and chemiluminescence research (35 papers)
Cited by: Biophysics Cellular and Molecular Neuroscience Structural Biology
Journals: Nature Science Cell
Partner nations: United States United Kingdom Germany

In The Last Decade

Roger Y. Tsien

318 papers receiving 108.4k citations

Hit Papers

5 papers align trajectories

What are hit papers?

Hit papers significantly outperform the citation benchmark for their cohort. A paper qualifies if it has ≥500 total citations, achieves ≥1.5× the top-1% citation threshold for papers in the same subfield and year (this is the minimum needed to enter the top 1%, not the average within it), or reaches the top citation threshold in at least one of its specific research topics.

A new generation of Ca2+ indicators with greatly improved fluorescence properties.

1985 19.8k citations Roger Y. Tsien et al. profile →
THE GREEN FLUORESCENT PROTEIN

1998 4.8k citations Roger Y. Tsien profile →
Fluorescent indicators for Ca2+based on green fluorescent proteins and calmodulin

1997 2.5k citations Roger Y. Tsien et al. profile →
A guide to choosing fluorescent proteins

2005 2.2k citations Nathan C. Shaner, Paul Steinbach et al. profile →
The Fluorescent Toolbox for Assessing Protein Location and Function

2006 2.2k citations Stephen Adams, Mark H. Ellisman et al. Science profile →
Calcium homeostasis in intact lymphocytes: cytoplasmic free calcium monitored with a new, intracellularly trapped fluorescent indicator.

1982 2.0k citations Roger Y. Tsien et al. The Journal of Cell Biology profile →
A monomeric red fluorescent protein

2002 2.0k citations Robert E. Campbell, Oded Tour et al. Proceedings of the National Academy of Sciences profile →
New calcium indicators and buffers with high selectivity against magnesium and protons: design, synthesis, and properties of prototype structures

1980 1.9k citations Roger Y. Tsien Biochemistry profile →
Partitioning of Lipid-Modified Monomeric GFPs into Membrane Microdomains of Live Cells

2002 1.9k citations David A. Zacharias, Jonathan D. Violin et al. Science profile →
Crystal Structure of the Aequorea victoria Green Fluorescent Protein

1996 1.8k citations Roger Y. Tsien, S. James Remington et al. Science profile →
Creating new fluorescent probes for cell biology

2002 1.6k citations Robert E. Campbell, Roger Y. Tsien et al. profile →
Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores

1989 1.4k citations Roger Y. Tsien et al. profile →
Specific Covalent Labeling of Recombinant Protein Molecules Inside Live Cells

1998 1.3k citations Stephen Adams, Roger Y. Tsien et al. Science profile →
Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer

1996 1.2k citations Roger Y. Tsien et al. profile →
Understanding, improving and using green fluorescent proteins

1995 1.1k citations Stephen Adams, Roger Y. Tsien et al. profile →
On/off blinking and switching behaviour of single molecules of green fluorescent protein

1997 1.0k citations Roger Y. Tsien et al. profile →
Identification of a signal for rapid export of proteins from the nucleus

1995 1.0k citations Roger Y. Tsien et al. profile →
Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins

1998 951 citations Roger Y. Tsien et al. Proceedings of the National Academy of Sciences profile →
Cytoplasmic pH and free Mg2+ in lymphocytes.

1982 917 citations Roger Y. Tsien et al. The Journal of Cell Biology profile →
A non-disruptive technique for loading calcium buffers and indicators into cells

1981 913 citations Roger Y. Tsien profile →
T-cell mitogens cause early changes in cytoplasmic free Ca2+ and membrane potential in lymphocytes

1982 910 citations Roger Y. Tsien et al. profile →
Reducing the Environmental Sensitivity of Yellow Fluorescent Protein

2001 840 citations Geoffrey S. Baird, Robert E. Campbell et al. profile →
Improving the photostability of bright monomeric orange and red fluorescent proteins

2008 778 citations Nathan C. Shaner, Michael Z. Lin et al. profile →
Investigating Mitochondrial Redox Potential with Redox-sensitive Green Fluorescent Protein Indicators

2004 774 citations Roger Y. Tsien, S. James Remington et al. profile →
Emptying of intracellular Ca2+ stores releases a novel small messenger that stimulates Ca2+ influx

1993 767 citations Roger Y. Tsien et al. profile →
New Biarsenical Ligands and Tetracysteine Motifs for Protein Labeling in Vitro and in Vivo: Synthesis and Biological Applications

2002 742 citations Stephen Adams, Robert E. Campbell et al. profile →
Cell-permeant caged InsP3 ester shows that Ca2+ spike frequency can optimize gene expression

1998 741 citations Michael Whitney, Roger Y. Tsien et al. profile →
Circular permutation and receptor insertion within green fluorescent proteins

1999 729 citations Geoffrey S. Baird, David A. Zacharias et al. Proceedings of the National Academy of Sciences profile →
Multicolor and Electron Microscopic Imaging of Connexin Trafficking

2002 723 citations Thomas J. Deerinck, Stephen Adams et al. Science profile →
Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral

2000 717 citations Geoffrey S. Baird, David A. Zacharias et al. Proceedings of the National Academy of Sciences profile →
Photochemically generated cytosolic calcium pulses and their detection by fluo-3

1989 716 citations Roger Y. Tsien et al. profile →
Engineering a memory with LTD and LTP

2014 700 citations Roger Y. Tsien et al. profile →
Tumor imaging by means of proteolytic activation of cell-penetrating peptides

2004 690 citations Tao Jiang, Emilia S. Olson et al. Proceedings of the National Academy of Sciences profile →
Fluorescent Sensors for Zn²⁺Based on a Fluorescein Platform: Synthesis, Properties and Intracellular Distribution

2001 678 citations Roger Y. Tsien et al. profile →
Dynamic and quantitative Ca ²⁺ measurements using improved cameleons

1999 675 citations Roger Y. Tsien et al. Proceedings of the National Academy of Sciences profile →
Changes in intramitochondrial and cytosolic pH: early events that modulate caspase activation during apoptosis

2000 645 citations Roger Y. Tsien et al. profile →
A Genetically Encoded Tag for Correlated Light and Electron Microscopy of Intact Cells, Tissues, and Organisms

2011 641 citations Xiaokun Shu, Varda Lev‐Ram et al. profile →
Imaging Dynamic Redox Changes in Mammalian Cells with Green Fluorescent Protein Indicators

2004 638 citations S. James Remington, Roger Y. Tsien et al. profile →
Na+/H+ exchange and cytoplasmic pH in the action of growth factors in human fibroblasts

1983 629 citations Roger Y. Tsien et al. profile →
Structural basis for dual excitation and photoisomerization of the Aequorea victoria green fluorescent protein

1997 593 citations Roger Y. Tsien, S. James Remington et al. Proceedings of the National Academy of Sciences profile →
Improving FRET dynamic range with bright green and red fluorescent proteins

2012 591 citations Mark J. Schnitzer, Roger Y. Tsien et al. profile →
ReaChR: a red-shifted variant of channelrhodopsin enables deep transcranial optogenetic excitation

2013 589 citations Roger Y. Tsien et al. profile →
Quantitation of Transcription and Clonal Selection of Single Living Cells with β-Lactamase as Reporter

1998 589 citations Michael Whitney, Roger Y. Tsien et al. Science profile →
Fluorescent indicators for cytosolic sodium

1989 562 citations Roger Y. Tsien et al. profile →
Fluorescence ratio imaging of cyclic AMP in single cells

1991 560 citations Stephen Adams, Roger Y. Tsien et al. profile →
Measurement of cytosolic free Ca2+ in individual small cells using fluorescence microscopy with dual excitation wavelengths

1985 560 citations Roger Y. Tsien et al. profile →
Calcium gradients in single smooth muscle cells revealed by the digital imaging microscope using Fura-2

1985 556 citations Roger Y. Tsien et al. profile →
Mammalian Expression of Infrared Fluorescent Proteins Engineered from a Bacterial Phytochrome

2009 556 citations Xiaokun Shu, Antoine Royant et al. Science profile →
Requirement for intrinsic protein tyrosine kinase in the immediate and late actions of the EGF receptor

1987 542 citations Roger Y. Tsien et al. profile →
Brominated 7-hydroxycoumarin-4-ylmethyls: Photolabile protecting groups with biologically useful cross-sections for two photon photolysis

1999 540 citations Roger Y. Tsien et al. Proceedings of the National Academy of Sciences profile →
Visualizing the mechanical activation of Src

2005 535 citations Yingxiao Wang, Roger Y. Tsien et al. profile →
Characterization of Engineered Channelrhodopsin Variants with Improved Properties and Kinetics

2009 525 citations Michael Z. Lin, Paul Steinbach et al. profile →
A New Cell-Permeable Fluorescent Probe for Zn²⁺

2000 524 citations Roger Y. Tsien et al. profile →
Fluorescent Probes of Cell Signaling

1989 521 citations Roger Y. Tsien profile →
Bcl-2-mediated alterations in endoplasmic reticulum Ca ²⁺ analyzed with an improved genetically encoded fluorescent sensor

2004 512 citations Amy E. Palmer, Roger Y. Tsien et al. Proceedings of the National Academy of Sciences profile →
Changes of free calcium levels with stages of the cell division cycle

1985 507 citations Roger Y. Tsien et al. profile →
Fas Preassociation Required for Apoptosis Signaling and Dominant Inhibition by Pathogenic Mutations

2000 504 citations David A. Zacharias, Roger Y. Tsien et al. Science profile →
The structure of the chromophore within DsRed, a red fluorescent protein from coral

2000 486 citations Geoffrey S. Baird, Roger Y. Tsien et al. Proceedings of the National Academy of Sciences profile →
Controlling Cell Chemistry with Caged Compounds

1993 481 citations Stephen Adams, Roger Y. Tsien profile →
Calcium Rises Abruptly and Briefly Throughout the Cell at the Onset of Anaphase

1986 478 citations Roger Y. Tsien et al. Science profile →
Genetically encoded reporters of protein kinase A activity reveal impact of substrate tethering

2001 474 citations Roger Y. Tsien et al. Proceedings of the National Academy of Sciences profile →
The Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins

2016 465 citations Robert E. Campbell, Michael Z. Lin et al. profile →
A genetically encoded fluorescent reporter reveals oscillatory phosphorylation by protein kinase C

2003 460 citations Jonathan D. Violin, Jin Zhang et al. The Journal of Cell Biology profile →
Fluorescence-guided surgery with live molecular navigation — a new cutting edge

2013 457 citations Quyen T. Nguyen, Roger Y. Tsien profile →
Cytoplasmic free Ca²⁺ in human platelets: Ca²⁺ thresholds and Ca‐independent activation for shape‐change and secretion

1982 454 citations Roger Y. Tsien et al. profile →
Cytosolic Ca2+ homeostasis in Ehrlich and Yoshida carcinomas. A new, membrane-permeant chelator of heavy metals reveals that these ascites tumor cell lines have normal cytosolic free Ca2+.

1985 441 citations Roger Y. Tsien et al. profile →
Spatially Resolved Dynamics of cAMP and Protein Kinase A Subunits in Aplysia Sensory Neurons

1993 433 citations Stephen Adams, Roger Y. Tsien et al. Science profile →
Neutral carrier ion-selective microelectrodes for measurement of intracellular free calcium

1980 419 citations Roger Y. Tsien et al. profile →
[14] Measurement of cytosolic free Ca2+ with quin2

1989 412 citations Roger Y. Tsien et al. profile →
Is Cytosolic Ionized Calcium Regulating Neutrophil Activation?

1983 411 citations Roger Y. Tsien et al. Science profile →
Ca2+ Indicators Based on Computationally Redesigned Calmodulin-Peptide Pairs

2006 405 citations Amy E. Palmer, Varda Lev‐Ram et al. profile →
Activity-dependent regulation of dendritic synthesis and trafficking of AMPA receptors

2004 401 citations Thomas J. Deerinck, Stephen Adams et al. profile →
Novel Chromophores and Buried Charges Control Color in mFruits ^,

2006 400 citations Xiaokun Shu, Nathan C. Shaner et al. Biochemistry profile →
A FlAsH-based FRET approach to determine G protein–coupled receptor activation in living cells

2005 393 citations Stephen Adams, Roger Y. Tsien et al. profile →
Aptamers Switch on Fluorescence of Triphenylmethane Dyes

2003 386 citations Stephen Adams, Roger Y. Tsien et al. profile →
A genetically encoded, fluorescent indicator for cyclic AMP in living cells

1999 385 citations Roger Y. Tsien et al. profile →
Genetically encoded fluorescent reporters of protein tyrosine kinase activities in living cells

2001 376 citations Roger Y. Tsien et al. Proceedings of the National Academy of Sciences profile →
Alteration of Stimulus-Specific Guard Cell Calcium Oscillations and Stomatal Closing in Arabidopsis det3 Mutant

2000 369 citations Roger Y. Tsien, Joanne Chory et al. Science profile →
Measuring calcium signaling using genetically targetable fluorescent indicators

2006 363 citations Amy E. Palmer, Roger Y. Tsien profile →
Coupling of Hormonal Stimulation and Transcription via the Cyclic AMP-Responsive Factor CREB Is Rate Limited by Nuclear Entry of Protein Kinase A

1993 354 citations Roger Y. Tsien et al. profile →
Mammalian cell–based optimization of the biarsenical-binding tetracysteine motif for improved fluorescence and affinity

2005 349 citations Stephen Adams, Roger Y. Tsien et al. profile →
Fluorescence ratio imaging: a new window into intracellular ionic signaling

1986 339 citations Roger Y. Tsien et al. profile →

Peers

Countries citing papers authored by Roger Y. Tsien

Since Specialization

Citations

This map shows the geographic impact of Roger Y. Tsien's research. It shows the number of citations coming from papers published by authors working in each country. You can also color the map by specialization and compare the number of citations received by Roger Y. Tsien with the expected number of citations based on a country's size and research output (numbers larger than one mean the country cites Roger Y. Tsien more than expected).

Fields of papers citing papers by Roger Y. Tsien

Since Specialization

Physical SciencesHealth SciencesLife SciencesSocial Sciences

This network shows the impact of papers produced by Roger Y. Tsien. Nodes represent research fields, and links connect fields that are likely to share authors. Colored nodes show fields that tend to cite the papers produced by Roger Y. Tsien. The network helps show where Roger Y. Tsien may publish in the future.

Co-authorship network of co-authors of Roger Y. Tsien

This figure shows the co-authorship network connecting the top 25 collaborators of Roger Y. Tsien. A scholar is included among the top collaborators of Roger Y. Tsien based on the total number of citations received by their joint publications. Widths of edges represent the number of papers authors have co-authored together. Node borders signify the number of papers an author published with Roger Y. Tsien. Roger Y. Tsien is excluded from the visualization to improve readability, since they are connected to all nodes in the network.

All Works

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20 of 20 papers shown

#	Work	Indexed citations
1	Do Perineuronal Nets Stabilize the Engram of a Synaptic Circuit? 2024 ·Cells ·Varda Lev‐Ram,(unknown),Thomas J. Deerinck,Eric A. Bushong,Álex Pérez,(unknown),Brandon H. Toyama,(unknown),Daniel B. McClatchy,Jeffrey N. Savas,Michael Whitney,Stephen Adams,Mark H. Ellisman,John R. Yates,Roger Y. Tsien	7
2	Impact of MMP-2 and MMP-9 enzyme activity on wound healing, tumor growth and RACPP cleavage 2018 ·PLoS ONE ·Dina V. Hingorani,(unknown),Jessica L. Crisp,Elamprakash N. Savariar,Jonathan Hasselmann,(unknown),Quyen T. Nguyen,Roger Y. Tsien,Michael Whitney,Lesley G. Ellies	40
3	Tumor Radiosensitization by Monomethyl Auristatin E: Mechanism of Action and Targeted Delivery 2015 ·Cancer Research ·(unknown),Elamprakash N. Savariar,Jessica L. Crisp,Karra A. Jones,(unknown),Daniel J. Scanderbeg,Quyen T. Nguyen,Jason K. Sicklick,Andrew M. Lowy,Roger Y. Tsien,Sunil J. Advani	68
4	The BRAIN Initiative: developing technology to catalyse neuroscience discovery. 2015 ·DSpace@MIT (Massachusetts Institute of Technology) ·Lyric A. Jorgenson,William T. Newsome,David J. Anderson,Cornelia I. Bargmann,Emery N. Brown,Karl Deisseroth,John P. Donoghue,Kathy Hudson,Geoffrey Ling,Peter R. MacLeish,Eve Marder,Richard A. Normann,Joshua R. Sanes,Mark J. Schnitzer,Terrence J. Sejnowski,David W. Tank,Roger Y. Tsien,Kâmil Uǧurbil,John C. Wingfield	0
5	Combined TP53 mutation/3p loss correlates with decreased radiosensitivity and increased matrix-metalloproteinase activity in head and neck carcinoma 2015 ·Oral Oncology ·(unknown),Samantha J. Hauff,(unknown),Ryan K. Orosco,Andrew M. Gross,Linda Nguyen,(unknown),William J. Moss,Michael Whitney,Ezra E.W. Cohen,Scott M. Lippman,Roger Y. Tsien,Trey Ideker,Sunil J. Advani,Quyen T. Nguyen	15
6	Dual Targeting of Integrin αvβ3 and Matrix Metalloproteinase-2 for Optical Imaging of Tumors and Chemotherapeutic Delivery 2014 ·Molecular Cancer Therapeutics ·Jessica L. Crisp,Elamprakash N. Savariar,(unknown),Lesley G. Ellies,Michael Whitney,Roger Y. Tsien	79
7	Gibberellins accumulate in the elongating endodermal cells of Arabidopsis root 2013 ·Proceedings of the National Academy of Sciences ·Eilon Shani,Roy Weinstain,Yi Zhang,Cristina Castillejo,Eirini Kaiserli,Joanne Chory,Roger Y. Tsien,Mark Estelle	160
8	Real-time In Vivo Molecular Detection of Primary Tumors and Metastases with Ratiometric Activatable Cell-Penetrating Peptides 2012 ·Cancer Research ·Elamprakash N. Savariar,Csilla N. Felsen,Nadia Nashi,Tao Jiang,Lesley G. Ellies,Paul Steinbach,Roger Y. Tsien,Quyen T. Nguyen	140
9	Activatable cell penetrating peptides linked to nanoparticles as dual probes for in vivo fluorescence and MR imaging of proteases 2010 ·Proceedings of the National Academy of Sciences ·Emilia S. Olson,Tao Jiang,Todd A. Aguilera,Quyen T. Nguyen,Lesley G. Ellies,Miriam Scadeng,Roger Y. Tsien	455
10	Simultaneous Visualization of Protumorigenic Src and MT1-MMP Activities with Fluorescence Resonance Energy Transfer 2010 ·Cancer Research ·Mingxing Ouyang,He Huang,Nathan C. Shaner,Albert G. Remacle,Sergey A. Shiryaev,Alex Y. Strongin,Roger Y. Tsien,Yingxiao Wang	91
11	Mammalian Expression of Infrared Fluorescent Proteins Engineered from a Bacterial Phytochromebreakdown → 2009 ·Science ·Xiaokun Shu,Antoine Royant,Michael Z. Lin,Todd A. Aguilera,Varda Lev‐Ram,Paul Steinbach,Roger Y. Tsien	556
12	A drug-controllable tag for visualizing newly synthesized proteins in cells and whole animals 2008 ·Proceedings of the National Academy of Sciences ·Michael Z. Lin,Jeffrey S. Glenn,Roger Y. Tsien	56
13	A hexahistidine-Zn ²⁺ -dye label reveals STIM1 surface exposure 2007 ·Proceedings of the National Academy of Sciences ·Christina Hauser,Roger Y. Tsien	134
14	Novel Chromophores and Buried Charges Control Color in mFruits ^,breakdown → 2006 ·Biochemistry ·Xiaokun Shu,Nathan C. Shaner,(unknown),Roger Y. Tsien,S. James Remington	400
15	Tumor imaging by means of proteolytic activation of cell-penetrating peptidesbreakdown → 2004 ·Proceedings of the National Academy of Sciences ·Tao Jiang,Emilia S. Olson,Quyen T. Nguyen,M. Roy,Patricia A. Jennings,Roger Y. Tsien	690
16	Evolution of new nonantibody proteins via iterative somatic hypermutation 2004 ·Proceedings of the National Academy of Sciences ·Lei Wang,(unknown),Paul Steinbach,Roger Y. Tsien	331
17	Imaging Tetrahymena ribozyme splicing activity in single live mammalian cells 2003 ·Proceedings of the National Academy of Sciences ·Sumitaka Hasegawa,(unknown),Roger Y. Tsien,Jianghong Rao	27
18	A genetically encoded fluorescent reporter reveals oscillatory phosphorylation by protein kinase Cbreakdown → 2003 ·The Journal of Cell Biology ·Jonathan D. Violin,Jin Zhang,Roger Y. Tsien,Alexandra C. Newton	460
19	A monomeric red fluorescent proteinbreakdown → 2002 ·Proceedings of the National Academy of Sciences ·Robert E. Campbell,Oded Tour,Amy E. Palmer,Paul Steinbach,Geoffrey S. Baird,David A. Zacharias,Roger Y. Tsien	1960
20	Partitioning of Lipid-Modified Monomeric GFPs into Membrane Microdomains of Live Cellsbreakdown → 2002 ·Science ·David A. Zacharias,Jonathan D. Violin,Alexandra C. Newton,Roger Y. Tsien	1851

About Roger Y. Tsien

Roger Y. Tsien is a scholar working on Biophysics, Cellular and Molecular Neuroscience and Structural Biology, having authored 321 papers that have together received 111.4k indexed citations. Recurring topics across this work include Advanced Fluorescence Microscopy Techniques (77 papers), Photoreceptor and optogenetics research (50 papers) and bioluminescence and chemiluminescence research (35 papers). The work is most often cited by research in Biophysics (20.0k citations), Cellular and Molecular Neuroscience (28.4k citations) and Structural Biology (1.6k citations). Roger Y. Tsien has collaborated with scholars based in United States, United Kingdom and Germany. Frequent co-authors include Martin Poenie, Grzegorz Grynkiewicz, Stephen Adams, T J Rink, Tullio Pozzan, David A. Zacharias, Paul Steinbach, Roger Heim, Geoffrey S. Baird and Joseph P. Y. Kao. Their work appears in journals such as Nature, Science and Cell.

Rankless uses publication and citation data sourced from OpenAlex, an open and comprehensive bibliographic database. While OpenAlex provides broad and valuable coverage of the global research landscape, it—like all bibliographic datasets—has inherent limitations. These include incomplete records, variations in author disambiguation, differences in journal indexing, and delays in data updates. As a result, some metrics and network relationships displayed in Rankless may not fully capture the entirety of a scholar's output or impact.

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