Michael W. Pfaffl

90.1k total citations · 9 hit papers
247 papers, 62.0k citations indexed

About

Michael W. Pfaffl is a scholar working on Molecular Biology, Cancer Research and Agronomy and Crop Science. According to data from OpenAlex, Michael W. Pfaffl has authored 247 papers receiving a total of 62.0k indexed citations (citations by other indexed papers that have themselves been cited), including 136 papers in Molecular Biology, 53 papers in Cancer Research and 46 papers in Agronomy and Crop Science. Recurrent topics in Michael W. Pfaffl's work include Molecular Biology Techniques and Applications (51 papers), MicroRNA in disease regulation (45 papers) and Extracellular vesicles in disease (38 papers). Michael W. Pfaffl is often cited by papers focused on Molecular Biology Techniques and Applications (51 papers), MicroRNA in disease regulation (45 papers) and Extracellular vesicles in disease (38 papers). Michael W. Pfaffl collaborates with scholars based in Germany, Switzerland and United Kingdom. Michael W. Pfaffl's co-authors include Vladimı́r Beneš, Stephen A. Bustin, Tania Nolan, Mikael Kubista, Aleš Tichopád, Christian Prgomet, Jo Vandesompele, Jan Hellemans, Jim F. Huggett and Carl T. Wittwer and has published in prestigious journals such as Proceedings of the National Academy of Sciences, Nucleic Acids Research and Nature Communications.

In The Last Decade

Michael W. Pfaffl

238 papers receiving 61.1k citations

Hit Papers

A new mathematical model for relative quantification in r... 2001 2026 2009 2017 2001 2009 2002 2004 2006 5.0k 10.0k 15.0k 20.0k 25.0k
What are hit papers?

Hit papers significantly outperform the citation benchmark for their cohort. A paper qualifies if it has ≥500 total citations, achieves ≥1.5× the top-1% citation threshold for papers in the same subfield and year (this is the minimum needed to enter the top 1%, not the average within it), or reaches the top citation threshold in at least one of its specific research topics.

  1. A new mathematical model for relative quantification in real-time RT-PCR
    2001 28.9k citations Michael W. Pfaffl Nucleic Acids Research profile →
  2. The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments
    2009 12.4k citations Michael W. Pfaffl et al. profile →
  3. Relative expression software tool (REST(C)) for group-wise comparison and statistical analysis of relative expression results in real-time PCR
    2002 6.5k citations Michael W. Pfaffl Nucleic Acids Research profile →
  4. Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper – Excel-based tool using pair-wise correlations
    2004 4.2k citations Michael W. Pfaffl, Aleš Tichopád et al. profile →
  5. RNA integrity and the effect on the real-time qRT-PCR performance
    2006 1.1k citations Michael W. Pfaffl et al. profile →
  6. Quantitative real-time RT-PCR – a perspective
    2005 1.0k citations Michael W. Pfaffl et al. profile →
  7. The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments
    2013 646 citations Michael W. Pfaffl et al. profile →
  8. How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments
    2015 414 citations Aleš Tichopád, Michael W. Pfaffl et al. profile →
  9. MIQE 2.0: Revision of the Minimum Information for Publication of Quantitative Real-Time PCR Experiments Guidelines
    2025 22 citations Michael W. Pfaffl et al. profile →

Peers — A (Enhanced Table)

Peers by citation overlap · career bar shows stage (early→late) cites · hero ref

Name h Career Trend Papers Cites
Michael W. Pfaffl Germany 47 30.7k 9.5k 7.1k 6.7k 6.1k 247 62.0k
Jun Wang China 107 34.0k 1.1× 11.7k 1.2× 5.4k 0.8× 9.5k 1.4× 7.9k 1.3× 1.9k 66.2k
Michael I. Love United States 31 39.1k 1.3× 11.3k 1.2× 8.8k 1.2× 7.0k 1.1× 8.9k 1.5× 93 68.1k
Christian von Mering Switzerland 68 45.6k 1.5× 7.0k 0.7× 5.6k 0.8× 6.3k 0.9× 7.5k 1.2× 134 69.7k
Lior Pachter United States 56 36.2k 1.2× 12.4k 1.3× 4.8k 0.7× 7.7k 1.2× 8.1k 1.3× 175 55.2k
Mark D. Robinson Switzerland 57 29.2k 1.0× 6.7k 0.7× 5.5k 0.8× 5.5k 0.8× 7.4k 1.2× 173 46.9k
Jo Vandesompele Belgium 70 29.8k 1.0× 5.6k 0.6× 4.5k 0.6× 5.3k 0.8× 11.2k 1.8× 299 50.4k
Daehwan Kim United States 8 28.6k 0.9× 14.3k 1.5× 4.6k 0.6× 6.1k 0.9× 6.9k 1.1× 12 48.0k
Simon Anders Germany 31 51.5k 1.7× 15.8k 1.7× 10.7k 1.5× 9.6k 1.4× 11.8k 1.9× 59 87.9k
Geo Pertea United States 20 29.3k 1.0× 13.8k 1.5× 4.3k 0.6× 6.9k 1.0× 7.9k 1.3× 28 48.0k
Ben Langmead United States 31 49.5k 1.6× 18.4k 1.9× 6.0k 0.8× 11.2k 1.7× 8.7k 1.4× 79 79.0k

Countries citing papers authored by Michael W. Pfaffl

Since Specialization
Citations

This map shows the geographic impact of Michael W. Pfaffl's research. It shows the number of citations coming from papers published by authors working in each country. You can also color the map by specialization and compare the number of citations received by Michael W. Pfaffl with the expected number of citations based on a country's size and research output (numbers larger than one mean the country cites Michael W. Pfaffl more than expected).

Fields of papers citing papers by Michael W. Pfaffl

Since Specialization
Physical SciencesHealth SciencesLife SciencesSocial Sciences

This network shows the impact of papers produced by Michael W. Pfaffl. Nodes represent research fields, and links connect fields that are likely to share authors. Colored nodes show fields that tend to cite the papers produced by Michael W. Pfaffl. The network helps show where Michael W. Pfaffl may publish in the future.

Co-authorship network of co-authors of Michael W. Pfaffl

This figure shows the co-authorship network connecting the top 25 collaborators of Michael W. Pfaffl. A scholar is included among the top collaborators of Michael W. Pfaffl based on the total number of citations received by their joint publications. Widths of edges represent the number of papers authors have co-authored together. Node borders signify the number of papers an author published with Michael W. Pfaffl. Michael W. Pfaffl is excluded from the visualization to improve readability, since they are connected to all nodes in the network.

All Works

20 of 20 papers shown
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Hartung, Franziska, Sonja Schindela, Veronika Mussack, et al.. (2024). Extracellular vesicle miRNAs drive aberrant macrophage responses in NSAID‐exacerbated respiratory disease. Allergy. 79(7). 1893–1907. 8 indexed citations
3.
Mussack, Veronika, et al.. (2024). Growth behavior and mRNA expression profiling during growth of IPEC-J2 cells. BMC Research Notes. 17(1). 154–154. 1 indexed citations
4.
Brandes, Florian, Benedikt Kirchner, Matthias Klein, et al.. (2023). Extensive blood transcriptome analysis reveals cellular signaling networks activated by circulating glycocalyx components reflecting vascular injury in COVID-19. Frontiers in Immunology. 14. 1129766–1129766. 9 indexed citations
5.
Brandes, Florian, Annekathrin M. Keiler, Benedikt Kirchner, et al.. (2023). Extracellular Vesicles and Endocannabinoid Signaling in Patients with COVID-19. Cannabis and Cannabinoid Research. 9(5). 1326–1338. 2 indexed citations
6.
Berisha, Bajram, et al.. (2023). Local Expression Dynamics of Various Adipokines during Induced Luteal Regression (Luteolysis) in the Bovine Corpus Luteum. Animals. 13(20). 3221–3221. 2 indexed citations
7.
Estrella, Nicole, et al.. (2021). Impact of elevated air temperature and drought on pollen characteristics of major agricultural grass species. PLoS ONE. 16(3). e0248759–e0248759. 9 indexed citations
8.
Schilling, Daniela, Sophie Dobiasch, Susanne Raulefs, et al.. (2020). The Emerging Role of miRNAs for the Radiation Treatment of Pancreatic Cancer. Cancers. 12(12). 3703–3703. 15 indexed citations
9.
Hermann, Stefanie, Florian Brandes, Benedikt Kirchner, et al.. (2020). Diagnostic potential of circulating cell‐free microRNAs for community‐acquired pneumonia and pneumonia‐related sepsis. Journal of Cellular and Molecular Medicine. 24(20). 12054–12064. 29 indexed citations
10.
Berisha, Bajram, Dieter Schams, Fred Sinowatz, Daniela Rodler, & Michael W. Pfaffl. (2020). Hypoxia‐inducible factor‐1alpha and nitric oxide synthases in bovine follicles close to ovulation and early luteal angiogenesis. Reproduction in Domestic Animals. 55(11). 1573–1584. 5 indexed citations
11.
Hermann, Stefanie, Dominik Buschmann, Benedikt Kirchner, et al.. (2019). Transcriptomic profiling of cell‐free and vesicular microRNAs from matched arterial and venous sera. Journal of Extracellular Vesicles. 8(1). 1670935–1670935. 26 indexed citations
12.
Buschmann, Dominik, Benedikt Kirchner, Stefanie Hermann, et al.. (2018). Evaluation of serum extracellular vesicle isolation methods for profiling miRNAs by next‐generation sequencing. Journal of Extracellular Vesicles. 7(1). 1481321–1481321. 193 indexed citations
13.
Berisha, Bajram, Dieter Schams, Daniela Rodler, Fred Sinowatz, & Michael W. Pfaffl. (2018). Changes in the expression of prostaglandin family members in bovine corpus luteum during the estrous cycle and pregnancy. Molecular Reproduction and Development. 85(7). 622–634. 14 indexed citations
14.
Serr, Isabelle, Rainer W. Fürst, Martin G. Scherm, et al.. (2016). miRNA92a targets KLF2 and the phosphatase PTEN signaling to promote human T follicular helper precursors in T1D islet autoimmunity. Proceedings of the National Academy of Sciences. 113(43). E6659–E6668. 48 indexed citations
15.
16.
Becker, Christiane, Irmgard Riedmaier, Martina Reiter, et al.. (2010). Effect of trenbolone acetate plus estradiol on transcriptional regulation of metabolism pathways in bovine liver. Hormone Molecular Biology and Clinical Investigation. 2(2). 257–265. 11 indexed citations
17.
Reiter, Martina, Aleš Tichopád, Irmgard Riedmaier, Michael W. Pfaffl, & Heinrich H.D. Meyer. (2009). Monitoring gene expression in muscle tissue of macaca fascicularis under the influence of testosterone and SARM. Hormone Molecular Biology and Clinical Investigation. 1(2). 73–79. 2 indexed citations
18.
Riedmaier, Irmgard, et al.. (2009). RNA-Qualitätskontrolle in der Genexpressionsanalytik. BIOspektrum. 15. 512–514. 1 indexed citations
19.
Pfaffl, Michael W., Stefanie Wittmann, Heinrich Meyer, & R.M. Bruckmaier. (2003). Gene Expression of Immunologically Important Factors in Blood Cells, Milk Cells, and Mammary Tissue of Cows. Journal of Dairy Science. 86(2). 538–545. 64 indexed citations
20.
Pfaffl, Michael W.. (2001). A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Research. 29(9). 45e–45. 28869 indexed citations breakdown →

Rankless uses publication and citation data sourced from OpenAlex, an open and comprehensive bibliographic database. While OpenAlex provides broad and valuable coverage of the global research landscape, it—like all bibliographic datasets—has inherent limitations. These include incomplete records, variations in author disambiguation, differences in journal indexing, and delays in data updates. As a result, some metrics and network relationships displayed in Rankless may not fully capture the entirety of a scholar's output or impact.

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