Standout Papers
Citation Impact
Citing Papers
Genome Editing Using Cas9 Nickases
2014
Individuality in gut microbiota composition is a complex polygenic trait shaped by multiple environmental and host genetic factors
2010 Standout
Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation
2014 StandoutScienceNobel
Computational Design of Self-Assembling Protein Nanomaterials with Atomic Level Accuracy
2012 StandoutScienceNobel
Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells
2013 StandoutScience
Reprogrammable CRISPR/Cas9-based system for inducing site-specific DNA methylation
2016
Transferrin receptor targeting by de novo sheet extension
2021 StandoutNobel
Computational design of closely related proteins that adopt two well-defined but structurally divergent folds
2020 StandoutNobel
Rationally engineered Cas9 nucleases with improved specificity
2015 StandoutScience
Microbiota-induced activation of epithelial IL-6 signaling links inflammasome-driven inflammation with transmissible cancer
2013
In vivo genome editing improves muscle function in a mouse model of Duchenne muscular dystrophy
2015 Science
Compartmentalized Control of Skin Immunity by Resident Commensals
2012 Science
Proinflammatory T-cell responses to gut microbiota promote experimental autoimmune encephalomyelitis
2010
Rapid and automated design of two-component protein nanomaterials using ProteinMPNN
2024 StandoutNobel
TLR9 Blockade Inhibits Activation of Diabetogenic CD8+ T Cells and Delays Autoimmune Diabetes
2010
A CRISPR/Cas9-Based System for Reprogramming Cell Lineage Specification
2014
Both TALENs and CRISPR/Cas9 directly target the HBB IVS2–654 (C > T) mutation in β-thalassemia-derived iPSCs
2015
CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity
2018 StandoutScienceNobel
Systematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editing
2016
DNA interrogation by the CRISPR RNA-guided endonuclease Cas9
2014 StandoutNatureNobel
Targeted and genome-wide sequencing reveal single nucleotide variations impacting specificity of Cas9 in human stem cells
2014
WU-CRISPR: characteristics of functional guide RNAs for the CRISPR/Cas9 system
2015
Precision cancer mouse models through genome editing with CRISPR-Cas9
2015
How specific is CRISPR/Cas9 really?
2015
A CRISPR-based approach for targeted DNA demethylation
2016
Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage
2016 StandoutNature
Intestinal Microbial Metabolism of Phosphatidylcholine and Cardiovascular Risk
2013 Standout
Computational Design of a Protein-Based Enzyme Inhibitor
2013 StandoutNobel
High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects
2016 StandoutNature
Editing the epigenome: technologies for programmable transcription and epigenetic modulation
2016
Gender Bias in Autoimmunity Is Influenced by Microbiota
2013
Comprehensive analysis of the specificity of transcription activator-like effector nucleases
2014
In Vitro Enzymology of Cas9
2014
NLRP6 Inflammasome Regulates Colonic Microbial Ecology and Risk for Colitis
2011 Standout
Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library
2013
Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA
2014 Standout
Classification and evolution of type II CRISPR-Cas systems
2014 StandoutNobel
Expanding the Biologist’s Toolkit with CRISPR-Cas9
2015 StandoutNobel
Genome-wide specificity of DNA binding, gene regulation, and chromatin remodeling by TALE- and CRISPR/Cas9-based transcriptional activators
2015
Broad specificity profiling of TALENs results in engineered nucleases with improved DNA-cleavage specificity
2014
Comparison of Cas9 activators in multiple species
2016
Supervised classification of human microbiota
2010
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
2014 StandoutNature
Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells
2015
Targeted Genome Editing in Human Cells Using CRISPR/Cas Nucleases and Truncated Guide RNAs
2014
Multistate Protein Design Using CLEVER and CLASSY
2013
Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects
2014
Engineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9
2015
CRISPR-Cas Systems: Prokaryotes Upgrade to Adaptive Immunity
2014
Principles for designing ordered protein assemblies
2012
Multiplex CRISPR/Cas9-based genome editing for correction of dystrophin mutations that cause Duchenne muscular dystrophy
2015
Accurate design of co-assembling multi-component protein nanomaterials
2014 StandoutNatureNobel
Targeted genomic rearrangements using CRISPR/Cas technology
2014
Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors
2015
The CRISPR/Cas9 System Facilitates Clearance of the Intrahepatic HBV Templates In Vivo
2014
Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease
2014 Nature
Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds
2014
Enabling functional genomics with genome engineering
2015
Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers
2015
Functional Repair of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients
2013
Highly efficient Cas9-mediated transcriptional programming
2015
Regulation of inflammatory responses by gut microbiota and chemoattractant receptor GPR43
2009 StandoutNature
sgRNAcas9: A Software Package for Designing CRISPR sgRNA and Evaluating Potential Off-Target Cleavage Sites
2014
Exploiting CRISPR–Cas immune systems for genome editing in bacteria
2015
Defining and improving the genome-wide specificities of CRISPR–Cas9 nucleases
2016
Protein folding and de novo protein design for biotechnological applications
2013
RNA-Guided Genome Editing in Plants Using a CRISPR–Cas System
2013
In Vitro Reconstitution and Crystallization of Cas9 Endonuclease Bound to a Guide RNA and a DNA Target
2015
Human nutrition, the gut microbiome and the immune system
2011 StandoutNature
Role of the Microbiota in Immunity and Inflammation
2014 Standout
CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes
2015
Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity
2013 Standout
Applications of CRISPR–Cas systems in neuroscience
2015
Enhanced proofreading governs CRISPR–Cas9 targeting accuracy
2017 StandoutNatureNobel
CHOPCHOP v2: a web tool for the next generation of CRISPR genome engineering
2016
Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach
2015
Improving CRISPR-Cas nuclease specificity using truncated guide RNAs
2014 Standout
A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging
2014
The coming of age of de novo protein design
2016 StandoutNatureNobel
CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing
2014
Oral tolerance
2011
Cas9 as a versatile tool for engineering biology
2013
A Broad-Spectrum Inhibitor of CRISPR-Cas9
2017 StandoutNobel
An iCRISPR Platform for Rapid, Multiplexable, and Inducible Genome Editing in Human Pluripotent Stem Cells
2014
A genome-wide analysis of Cas9 binding specificity using ChIP-seq and targeted sequence capture
2015
Enhanced Specificity and Efficiency of the CRISPR/Cas9 System with Optimized sgRNA Parameters in Drosophila
2014
Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells
2014
The Impact of the Gut Microbiota on Human Health: An Integrative View
2012 Standout
Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification
2014
In vivo genome editing using Staphylococcus aureus Cas9
2015 StandoutNature
Photoactivatable CRISPR-Cas9 for optogenetic genome editing
2015
One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated Genome Engineering
2013
CRISPR-mediated direct mutation of cancer genes in the mouse liver
2014 Nature
Cas9-chromatin binding information enables more accurate CRISPR off-target prediction
2015
Taking dendritic cells into medicine
2007 StandoutNatureNobel
CRISPR-Cas systems for editing, regulating and targeting genomes
2014 Standout
Genome engineering using the CRISPR-Cas9 system
2013 Standout
CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences
2014
High-throughput functional genomics using CRISPR–Cas9
2015
Development and Applications of CRISPR-Cas9 for Genome Engineering
2014 Standout
Enantioselective Enzymes by Computational Design and In Silico Screening
2015 StandoutNobel
Functional genetics for all: engineered nucleases, CRISPR and the gene editing revolution
2014
Practical approaches to designing novel protein assemblies
2013
Engineered CRISPR-Cas9 nucleases with altered PAM specificities
2015 Nature
DNA-binding-domain fusions enhance the targeting range and precision of Cas9
2015
Applications of CRISPR technologies in research and beyond
2016 StandoutNobel
A thermostable Cas9 with increased lifetime in human plasma
2017 StandoutNobel
Emerging themes in the computational design of novel enzymes and protein–protein interfaces
2012
Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease
2014
GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases
2014 Standout
Genetics and pathogenesis of inflammatory bowel disease
2011 StandoutNature
Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA
2016
Intestinal microbiota, evolution of the immune system and the bad reputation of pro-inflammatory immunity
2011
A light-inducible CRISPR-Cas9 system for control of endogenous gene activation
2015
Lessons from Enzyme Kinetics Reveal Specificity Principles for RNA-Guided Nucleases in RNA Interference and CRISPR-Based Genome Editing
2017
Genome Editing in Human Stem Cells
2014
Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins
2014
CRISPR-Based Technologies for the Manipulation of Eukaryotic Genomes
2016 Standout
Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases
2013
Intestinal microbiota: Shaping local and systemic immune responses
2011
Compartmentalized and systemic control of tissue immunity by commensals
2013
Computational enzyme design: Transitioning from catalytic proteins to enzymes
2014
Small molecule–triggered Cas9 protein with improved genome-editing specificity
2015
Metagenomics and Personalized Medicine
2011
Efficient genome editing in the mouse brain by local delivery of engineered Cas9 ribonucleoprotein complexes
2017 StandoutNobel
Orthogonal Cas9 proteins for RNA-guided gene regulation and editing
2013
Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection
2016 StandoutNatureNobel
Low Incidence of Off-Target Mutations in Individual CRISPR-Cas9 and TALEN Targeted Human Stem Cell Clones Detected by Whole-Genome Sequencing
2014
Adapting CRISPR/Cas9 for Functional Genomics Screens
2014
Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9
2016 StandoutNobel
Scientific Benchmarks for Guiding Macromolecular Energy Function Improvement
2013 StandoutNobel
An accurate binding interaction model in de novo computational protein design of interactions: If you build it, they will bind
2013
Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases
2014
Innate receptors and microbes in induction of autoimmunity
2009
RNA-dependent DNA endonuclease Cas9 of the CRISPR system: Holy Grail of genome editing?
2013
Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing
2014
Pharmacological inhibition of DNA-PK stimulates Cas9-mediated genome editing
2015
Dysregulation of Allergic Airway Inflammation in the Absence of Microbial Colonization
2011
Dimeric CRISPR RNA-Guided FokI-dCas9 Nucleases Directed by Truncated gRNAs for Highly Specific Genome Editing
2015
Delivery and Specificity of CRISPR/Cas9 Genome Editing Technologies for Human Gene Therapy
2015
The Bacterial Origins of the CRISPR Genome-Editing Revolution
2015
Protospacer Adjacent Motif (PAM)-Distal Sequences Engage CRISPR Cas9 DNA Target Cleavage
2014
Accurate design of megadalton-scale two-component icosahedral protein complexes
2016 StandoutScienceNobel
Genome Engineering with Targetable Nucleases
2014
Structure of a designed tetrahedral protein assembly variant engineered to have improved soluble expression
2015 StandoutNobel
CD4+CD25−LAG3+regulatory T cells controlled by the transcription factor Egr-2
2009 StandoutNobel
Concerning RNA-guided gene drives for the alteration of wild populations
2014
Biallelic genome modification in F0Xenopus tropicalis embryos using the CRISPR/Cas system
2013
Interactions Between the Microbiota and the Immune System
2012 StandoutScience
Understanding the role of gut microbiome–host metabolic signal disruption in health and disease
2011
The chemistry of Cas9 and its CRISPR colleagues
2017 StandoutNobel
The new frontier of genome engineering with CRISPR-Cas9
2014 StandoutScienceNobel
Succession of microbial consortia in the developing infant gut microbiome
2010 Standout
Constructing arrays of proteins
2013
De novo design of protein homo-oligomers with modular hydrogen-bond network–mediated specificity
2016 StandoutScienceNobel
CasOT: a genome-wide Cas9/gRNA off-target searching tool
2014
Protein design: Past, present, and future
2015
Off‐target assessment of CRISPR ‐C as9 guiding RNA s in human iPS and mouse ES cells
2014
Toll-Like Receptor–Gut Microbiota Interactions: Perturb at Your Own Risk!
2011
Computational design and experimental verification of a symmetric protein homodimer
2015
Identification of potential drug targets for tuberous sclerosis complex by synthetic screens combining CRISPR-based knockouts with RNAi
2015
Metagenomics of the Human Body
2010
Naturally transmitted segmented filamentous bacteria segregate with diabetes protection in nonobese diabetic mice
2011
Diet-Induced Metabolic Improvements in a Hamster Model of Hypercholesterolemia Are Strongly Linked to Alterations of the Gut Microbiota
2009
From Structure to Function: the Ecology of Host-Associated Microbial Communities
2010
Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells
2013
Orthogonal gene knockout and activation with a catalytically active Cas9 nuclease
2015
A general strategy to construct small molecule biosensors in eukaryotes
2015 StandoutNobel
Toward defining the autoimmune microbiome for type 1 diabetes
2010
Fast UniFrac: facilitating high-throughput phylogenetic analyses of microbial communities including analysis of pyrosequencing and PhyloChip data
2009
Target specificity of the CRISPR‐Cas9 system
2014
Efficient generation of genetically distinct pigs in a single pregnancy using multiplexed single‐guide RNA and carbohydrate selection
2014
Phylogeny of Cas9 determines functional exchangeability of dual-RNA and Cas9 among orthologous type II CRISPR-Cas systems
2013 StandoutNobel
Microbiota regulates immune defense against respiratory tract influenza A virus infection
2011 Standout
Combined Covalent-Electrostatic Model of Hydrogen Bonding Improves Structure Prediction with Rosetta
2015 StandoutNobel
Reconfigurable asymmetric protein assemblies through implicit negative design
2022 StandoutScienceNobel
Unravelling the effects of the environment and host genotype on the gut microbiome
2011
Successful Transmission of a Retrovirus Depends on the Commensal Microbiota
2011 Science
Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery
2014 StandoutNobel
Works of P. Benjamin Stranges being referenced
A Generic Program for Multistate Protein Design
2011
A comparison of successful and failed protein interface designs highlights the challenges of designing buried hydrogen bonds
2012
CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering
2013
Elimination of Antigen-Presenting Cells and Autoreactive T Cells by Fas Contributes to Prevention of Autoimmunity
2007
Innate immunity and intestinal microbiota in the development of Type 1 diabetes
2008 StandoutNature
Computational design of a symmetric homodimer using β-strand assembly
2011