Standout Papers
Citation Impact
Citing Papers
Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation
2014 StandoutScienceNobel
Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells
2013 StandoutScience
RNA-directed gene editing specifically eradicates latent and prevents new HIV-1 infection
2014
Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage
2016 StandoutScienceNobel
CRISPR/Cas9 in Genome Editing and Beyond
2016
High-throughput biochemical profiling reveals sequence determinants of dCas9 off-target binding and unbinding
2017 StandoutNobel
In vivo gene editing in dystrophic mouse muscle and muscle stem cells
2015 Science
Multiplex Genome-Edited T-cell Manufacturing Platform for “Off-the-Shelf” Adoptive T-cell Immunotherapies
2015
Efficient CRISPR-mediated mutagenesis in primary immune cells using CrispRGold and a C57BL/6 Cas9 transgenic mouse line
2016
Rationally engineered Cas9 nucleases with improved specificity
2015 StandoutScience
CRISPR/Cas9 cleavage of viral DNA efficiently suppresses hepatitis B virus
2015 StandoutNatureNobel
The iCRISPR Platform for Rapid Genome Editing in Human Pluripotent Stem Cells
2014
The crystal structure of Cpf1 in complex with CRISPR RNA
2016 Nature
Myosin-II mediated traction forces evoke localized Piezo1-dependent Ca2+ flickers
2019
CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity
2018 StandoutScienceNobel
Systematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editing
2016
Genome-scale measurement of off-target activity using Cas9 toxicity in high-throughput screens
2017
DNA interrogation by the CRISPR RNA-guided endonuclease Cas9
2014 StandoutNatureNobel
Targeted and genome-wide sequencing reveal single nucleotide variations impacting specificity of Cas9 in human stem cells
2014
A pre-screening FISH-based method to detect CRISPR/Cas9 off-targets in mouse embryonic stem cells
2015
CRISPR/Cas9-mediated targeted chromosome elimination
2017
WU-CRISPR: characteristics of functional guide RNAs for the CRISPR/Cas9 system
2015
Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system
2014
Precision cancer mouse models through genome editing with CRISPR-Cas9
2015
How specific is CRISPR/Cas9 really?
2015
Structure and Engineering of Francisella novicida Cas9
2016
The promise and challenge of therapeutic genome editing
2020 StandoutNatureNobel
Precise Correction of the Dystrophin Gene in Duchenne Muscular Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and CRISPR-Cas9
2014 Nobel
A CRISPR-based approach for targeted DNA demethylation
2016
MAGeCK enables robust identification of essential genes from genome-scale CRISPR/Cas9 knockout screens
2014 Standout
CRISPR-based technologies: prokaryotic defense weapons repurposed
2014
Genome-wide CRISPR Screen in a Mouse Model of Tumor Growth and Metastasis
2015
CRISPR/Cas9-mediated gene knockout is insensitive to target copy number but is dependent on guide RNA potency and Cas9/sgRNA threshold expression level
2017
CRISPR/Cas9 genome editing in human hematopoietic stem cells
2018
Discoveries in structure and physiology of mechanically activated ion channels
2020 StandoutNatureNobel
CRISPR-Cas9 cleavage efficiency correlates strongly with target-sgRNA folding stability: from physical mechanism to off-target assessment
2017
Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System
2015 Standout
High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects
2016 StandoutNature
Precise Gene Editing Preserves Hematopoietic Stem Cell Function following Transient p53-Mediated DNA Damage Response
2019
PAM multiplicity marks genomic target sites as inhibitory to CRISPR-Cas9 editing
2015
A multifunctional AAV–CRISPR–Cas9 and its host response
2016
Editing the epigenome: technologies for programmable transcription and epigenetic modulation
2016
Accurate classification of BRCA1 variants with saturation genome editing
2018 Nature
Saturation variant interpretation using CRISPR prime editing
2022
CRISPR/Cas9 β-globin gene targeting in human haematopoietic stem cells
2016 Nature
Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library
2013
Landscape of target:guide homology effects on Cas9-mediated cleavage
2014 Nobel
Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA
2014 Standout
Comparison of non-canonical PAMs for CRISPR/Cas9-mediated DNA cleavage in human cells
2014
CD22 blockade restores homeostatic microglial phagocytosis in ageing brains
2019 StandoutNatureNobel
Efficient and Allele-Specific Genome Editing of Disease Loci in Human iPSCs
2014
Expanding the Biologist’s Toolkit with CRISPR-Cas9
2015 StandoutNobel
Genome-wide specificity of DNA binding, gene regulation, and chromatin remodeling by TALE- and CRISPR/Cas9-based transcriptional activators
2015
Induced Pluripotent Stem Cells Meet Genome Editing
2016
Prediction of off-target activities for the end-to-end design of CRISPR guide RNAs
2018
Sequence determinants of improved CRISPR sgRNA design
2015
CRISPR-Cas12a has widespread off-target and dsDNA-nicking effects
2020
p53 inhibits CRISPR–Cas9 engineering in human pluripotent stem cells
2018
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
2014 StandoutNature
Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells
2015
AAV-Mediated Delivery of Zinc Finger Nucleases Targeting Hepatitis B Virus Inhibits Active Replication
2014
Targeted Genome Editing in Human Cells Using CRISPR/Cas Nucleases and Truncated Guide RNAs
2014
Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects
2014
Expanding the CRISPR imaging toolset withStaphylococcus aureusCas9 for simultaneous imaging of multiple genomic loci
2016
Engineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9
2015
Genome editing of CXCR4 by CRISPR/cas9 confers cells resistant to HIV-1 infection
2015
Methods for Optimizing CRISPR-Cas9 Genome Editing Specificity
2016
New CRISPR–Cas systems from uncultivated microbes
2016 StandoutNatureNobel
NmeCas9 is an intrinsically high-fidelity genome-editing platform
2018
Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection
2015
Saturation editing of genomic regions by multiplex homology-directed repair
2014 Nature
Multiplex CRISPR/Cas9-based genome editing for correction of dystrophin mutations that cause Duchenne muscular dystrophy
2015
Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors
2015
Multiplex gene editing by CRISPR–Cpf1 using a single crRNA array
2016
Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease
2014 Nature
Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation
2014 Standout
Functional Repair of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients
2013
Correction of a Genetic Disease in Mouse via Use of CRISPR-Cas9
2013
A machine learning approach for predicting CRISPR-Cas9 cleavage efficiencies and patterns underlying its mechanism of action
2017
An Efficient Genotyping Method for Genome-modified Animals and Human Cells Generated with CRISPR/Cas9 System
2014
CCTop: An Intuitive, Flexible and Reliable CRISPR/Cas9 Target Prediction Tool
2015
A split-Cas9 architecture for inducible genome editing and transcription modulation
2015
sgRNAcas9: A Software Package for Designing CRISPR sgRNA and Evaluating Potential Off-Target Cleavage Sites
2014
Genome editing reveals a role for OCT4 in human embryogenesis
2017 Nature
Defining and improving the genome-wide specificities of CRISPR–Cas9 nucleases
2016
RNA-Guided Genome Editing in Plants Using a CRISPR–Cas System
2013
A Cas9 Ribonucleoprotein Platform for Functional Genetic Studies of HIV-Host Interactions in Primary Human T Cells
2016 StandoutNobel
CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes
2015
Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity
2013 Standout
Enhanced proofreading governs CRISPR–Cas9 targeting accuracy
2017 StandoutNatureNobel
CHOPCHOP v2: a web tool for the next generation of CRISPR genome engineering
2016
The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA
2016 StandoutNatureNobel
Streptococcus thermophilus CRISPR-Cas9 Systems Enable Specific Editing of the Human Genome
2015
CRISPR interference-based specific and efficient gene inactivation in the brain
2018
BLISS is a versatile and quantitative method for genome-wide profiling of DNA double-strand breaks
2017
Sequence features associated with the cleavage efficiency of CRISPR/Cas9 system
2016
Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9
2016 Standout
Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch
2016 StandoutNobel
Improved Cell-Penetrating Zinc-Finger Nuclease Proteins for Precision Genome Engineering
2015
Improving CRISPR-Cas nuclease specificity using truncated guide RNAs
2014 Standout
Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA
2014
Genome editing by natural and engineered CRISPR-associated nucleases
2018
A Biophysical Model of CRISPR/Cas9 Activity for Rational Design of Genome Editing and Gene Regulation
2016
Massively parallel determination and modeling of endonuclease substrate specificity
2014 StandoutNobel
CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing
2014
Cas9 as a versatile tool for engineering biology
2013
Crystal Structure of Staphylococcus aureus Cas9
2015
An iCRISPR Platform for Rapid, Multiplexable, and Inducible Genome Editing in Human Pluripotent Stem Cells
2014
Programmable RNA recognition and cleavage by CRISPR/Cas9
2014 StandoutNatureNobel
A genome-wide analysis of Cas9 binding specificity using ChIP-seq and targeted sequence capture
2015
Enhanced Specificity and Efficiency of the CRISPR/Cas9 System with Optimized sgRNA Parameters in Drosophila
2014
Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system
2013
Beyond editing: repurposing CRISPR–Cas9 for precision genome regulation and interrogation
2015
Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification
2014
Targeted activation of diverse CRISPR-Cas systems for mammalian genome editing via proximal CRISPR targeting
2017
In vivo genome editing using Staphylococcus aureus Cas9
2015 StandoutNature
A CRISPR-Cas9 gene drive system targeting female reproduction in the malaria mosquito vector Anopheles gambiae
2015
Photoactivatable CRISPR-Cas9 for optogenetic genome editing
2015
One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated Genome Engineering
2013
Seamless gene correction of β-thalassemia mutations in patient-specific iPSCs using CRISPR/Cas9 and piggyBac
2014
CRISPR-mediated direct mutation of cancer genes in the mouse liver
2014 Nature
Cas9-chromatin binding information enables more accurate CRISPR off-target prediction
2015
CRISPR-Cas systems for editing, regulating and targeting genomes
2014 Standout
Genome engineering using the CRISPR-Cas9 system
2013 Standout
Discovery of cancer drug targets by CRISPR-Cas9 screening of protein domains
2015
Nuclear domain ‘knock-in’ screen for the evaluation and identification of small molecule enhancers of CRISPR-based genome editing
2015
CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences
2014
High-throughput functional genomics using CRISPR–Cas9
2015
Development and Applications of CRISPR-Cas9 for Genome Engineering
2014 Standout
Conformational control of DNA target cleavage by CRISPR–Cas9
2015 StandoutNatureNobel
De novo design of a fluorescence-activating β-barrel
2018 StandoutNatureNobel
Massively Parallel Biophysical Analysis of CRISPR-Cas Complexes on Next Generation Sequencing Chips
2017
Engineered CRISPR-Cas9 nucleases with altered PAM specificities
2015 Nature
Rescue of high-specificity Cas9 variants using sgRNAs with matched 5’ nucleotides
2017
CRISPR/Cas9 cleavage of viral DNA efficiently suppresses hepatitis B virus
2015 StandoutNobel
The next generation of CRISPR–Cas technologies and applications
2019
Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation
2014
Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease
2014
Imaging Genomic Elements in Living Cells Using CRISPR/Cas9
2014
GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases
2014 Standout
Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System
2013 Nobel
The initiation, propagation and dynamics of CRISPR-SpyCas9 R-loop complex
2017
In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9
2014
Reactivating Fetal Hemoglobin Expression in Human Adult Erythroblasts Through BCL11A Knockdown Using Targeted Endonucleases
2016
CRISPR-Cas9 genome editing induces megabase-scale chromosomal truncations
2019
Efficient introduction of specific homozygous and heterozygous mutations using CRISPR/Cas9
2016 Nature
Targeted DNA demethylation in vivo using dCas9–peptide repeat and scFv–TET1 catalytic domain fusions
2016
Genome Editing in Human Stem Cells
2014
Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins
2014
CRISPR-Cas9-induced t(11;19)/MLL-ENL translocations initiate leukemia in human hematopoietic progenitor cells in vivo
2017 StandoutNobel
Directed evolution of CRISPR-Cas9 to increase its specificity
2018
Crystal Structure of Cpf1 in Complex with Guide RNA and Target DNA
2016
CRISPR-Based Technologies for the Manipulation of Eukaryotic Genomes
2016 Standout
Functional Correction of Large Factor VIII Gene Chromosomal Inversions in Hemophilia A Patient-Derived iPSCs Using CRISPR-Cas9
2015
RS-1 enhances CRISPR/Cas9- and TALEN-mediated knock-in efficiency
2016
Cornerstones of CRISPR–Cas in drug discovery and therapy
2016 StandoutNobel
Engineering human tumour-associated chromosomal translocations with the RNA-guided CRISPR–Cas9 system
2014
Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases
2013
Rapid modelling of cooperating genetic events in cancer through somatic genome editing
2014 Nature
Heritable Multiplex Genetic Engineering in Rats Using CRISPR/Cas9
2014
CasX enzymes comprise a distinct family of RNA-guided genome editors
2019 StandoutNatureNobel
Small molecule–triggered Cas9 protein with improved genome-editing specificity
2015
Kinetic Basis for DNA Target Specificity of CRISPR-Cas12a
2018
Lysosome-targeting chimaeras for degradation of extracellular proteins
2020 StandoutNatureNobel
Human Germline Genome Editing
2017
Genotyping with CRISPR-Cas-derived RNA-guided endonucleases
2014
Efficient genome editing in the mouse brain by local delivery of engineered Cas9 ribonucleoprotein complexes
2017 StandoutNobel
Protein Engineering of Cas9 for Enhanced Function
2014
Refined sgRNA efficacy prediction improves large- and small-scale CRISPR–Cas9 applications
2017 StandoutNobel
GuideScan software for improved single and paired CRISPR guide RNA design
2017
Small Molecules Enhance CRISPR Genome Editing in Pluripotent Stem Cells
2015
Partial DNA-guided Cas9 enables genome editing with reduced off-target activity
2018
Efficient delivery of nuclease proteins for genome editing in human stem cells and primary cells
2015
DNase H Activity of Neisseria meningitidis Cas9
2015
Distinct patterns of Cas9 mismatch tolerancein vitroandin vivo
2016 Nobel
CCR5 Gene Disruption via Lentiviral Vectors Expressing Cas9 and Single Guided RNA Renders Cells Resistant to HIV-1 Infection
2014
Low Incidence of Off-Target Mutations in Individual CRISPR-Cas9 and TALEN Targeted Human Stem Cell Clones Detected by Whole-Genome Sequencing
2014
Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9
2016 StandoutNobel
Structure and specificity of the RNA-guided endonuclease Cas9 during DNA interrogation, target binding and cleavage
2015
Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases
2014
Efficient Mutagenesis by Cas9 Protein-Mediated Oligonucleotide Insertion and Large-Scale Assessment of Single-Guide RNAs
2014
RNA-dependent DNA endonuclease Cas9 of the CRISPR system: Holy Grail of genome editing?
2013
Progress and prospects in plant genome editing
2017
Massively parallel kinetic profiling of natural and engineered CRISPR nucleases
2020 StandoutNobel
Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing
2014
Pharmacological inhibition of DNA-PK stimulates Cas9-mediated genome editing
2015
Genome-editing revolution: My whirlwind year with CRISPR
2015 StandoutNatureNobel
Dimeric CRISPR RNA-Guided FokI-dCas9 Nucleases Directed by Truncated gRNAs for Highly Specific Genome Editing
2015
Delivery and Specificity of CRISPR/Cas9 Genome Editing Technologies for Human Gene Therapy
2015
Fanconi Anemia Gene Editing by the CRISPR/Cas9 System
2014
Protospacer Adjacent Motif (PAM)-Distal Sequences Engage CRISPR Cas9 DNA Target Cleavage
2014
Genome Engineering with Targetable Nucleases
2014
A prudent path forward for genomic engineering and germline gene modification
2015 StandoutScienceNobel
Performance of the Cas9 Nickase System inDrosophila melanogaster
2014
GT-Scan: identifying unique genomic targets
2014
A Cas9–guide RNA complex preorganized for target DNA recognition
2015 StandoutScienceNobel
Dynamics of CRISPR-Cas9 genome interrogation in living cells
2015 StandoutScienceNobel
CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites
2014 Standout
Inhibition of HIV-1 infection of primary CD4+ T-cells by gene editing of CCR5 using adenovirus-delivered CRISPR/Cas9
2015
Selection of chromosomal DNA libraries using a multiplex CRISPR system
2014
Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases
2014 Standout
Concerning RNA-guided gene drives for the alteration of wild populations
2014
CRISPR-Cas: New Tools for Genetic Manipulations from Bacterial Immunity Systems
2015
Biallelic genome modification in F0Xenopus tropicalis embryos using the CRISPR/Cas system
2013
Genomes in Focus: Development and Applications of CRISPR‐Cas9 Imaging Technologies
2017 StandoutNobel
The chemistry of Cas9 and its CRISPR colleagues
2017 StandoutNobel
The new frontier of genome engineering with CRISPR-Cas9
2014 StandoutScienceNobel
Selection-free genome editing of the sickle mutation in human adult hematopoietic stem/progenitor cells
2016
CRISPR-Cas9 nuclear dynamics and target recognition in living cells
2016
Imaging Specific Genomic DNA in Living Cells
2016
CRISPR-CasΦ from huge phages is a hypercompact genome editor
2020 StandoutScienceNobel
CasOT: a genome-wide Cas9/gRNA off-target searching tool
2014
Programmed DNA destruction by miniature CRISPR-Cas14 enzymes
2018 StandoutScienceNobel
Reprogramming homing endonuclease specificity through computational design and directed evolution
2013 StandoutNobel
Off‐target assessment of CRISPR ‐C as9 guiding RNA s in human iPS and mouse ES cells
2014
RNA-dependent RNA targeting by CRISPR-Cas9
2018 StandoutNobel
In vivo genome editing improves motor function and extends survival in a mouse model of ALS
2017
A New Class of Medicines through DNA Editing
2019
Rational design of a split-Cas9 enzyme complex
2015 StandoutNobel
Off-target predictions in CRISPR-Cas9 gene editing using deep learning
2018
Optical Control of CRISPR/Cas9 Gene Editing
2015
megaTALs: a rare-cleaving nuclease architecture for therapeutic genome engineering
2013 StandoutNobel
Evaluating and Enhancing Target Specificity of Gene-Editing Nucleases and Deaminases
2019
Overview of guide RNA design tools for CRISPR-Cas9 genome editing technology
2015
Exploiting CRISPR /C as systems for biotechnology
2013
Correction of diverse muscular dystrophy mutations in human engineered heart muscle by single-site genome editing
2018
Target specificity of the CRISPR‐Cas9 system
2014
CRISPR-Cas guides the future of genetic engineering
2018 StandoutScienceNobel
CASFISH: CRISPR/Cas9-mediated in situ labeling of genomic loci in fixed cells
2015
Accurate proteome-wide missense variant effect prediction with AlphaMissense
2023 StandoutScienceNobel
CRISPR/Cas9-Mediated Targeted Mutagenesis in the Liverwort Marchantia polymorpha L.
2014
Domain-focused CRISPR screen identifies HRI as a fetal hemoglobin regulator in human erythroid cells
2018 Science
Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery
2014 StandoutNobel
Works of Eli J. Fine being referenced
Quantifying Genome-Editing Outcomes at Endogenous Loci with SMRT Sequencing
2014
DNA targeting specificity of RNA-guided Cas9 nucleases
2013 Standout
CRISPR/Cas9 systems targeting β-globin and CCR5 genes have substantial off-target activity
2013
COSMID: A Web-based Tool for Identifying and Validating CRISPR/Cas Off-target Sites
2014
Quantifying on- and off-target genome editing
2015
TALENs facilitate targeted genome editing in human cells with high specificity and low cytotoxicity
2014
Trans-spliced Cas9 allows cleavage of HBB and CCR5 genes in human cells using compact expression cassettes
2015
An online bioinformatics tool predicts zinc finger and TALE nuclease off-target cleavage
2013